| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055885 |
| Chromosome: | chromosome 7 |
| Location: | 3829621 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338650 | POLI1,CSC3,POLH1 | Chlamydomonadale-specific family C protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAGGAGCGCGTATGCCAGGAGCATGGCGGTGGGCAGTAGCGGGTCAC |
| Internal bar code: | GCTGTATGTAGTATGTAATCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2743 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCACACTCAGGTTTGCA |
| Suggested primer 2: | GTATCCCGACACATAGCGCA |