| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055893 |
| Chromosome: | chromosome 3 |
| Location: | 4173721 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173200 | ERV2,TOX2,ERV1B | Essential for Respiration and Viability 1; (1 of 2) K17783 - mitochondrial FAD-linked sulfhydryl oxidase [EC:1.8.3.2] (ERV1, GFER, ALR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCGGGGACGGCCCAGCGGGCTGCACCTCCCTGCGACACGTGCGACAG |
| Internal bar code: | TTTTGCAGATATAAGGGGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3657 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGGGGATGAAGGGGGAG |
| Suggested primer 2: | CAGTTGAACACAGGCTTGCC |