| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055896 |
| Chromosome: | chromosome 16 |
| Location: | 4777992 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687750 | GT90F28,GT90-28 | GT90 family protein 28; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCGCGAGCGCGATGGGCACGTGTCCGAATTTAAATGTCGTGCTTGGA |
| Internal bar code: | GCGGCTGACGTCTTTAGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3144 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGCAGGAACCTGAGAT |
| Suggested primer 2: | CTTGCCCACTCAAGTAGCCA |