| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055918 |
| Chromosome: | chromosome 3 |
| Location: | 8280515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199500 | FAP129 | Flagellar Associated Protein 129 | CDS |
| Cre03.g199550 | (1 of 1) IPR000504//IPR012677//IPR014720 - RNA recognition motif domain // Nucleotide-binding alpha-beta plait domain // Double-stranded RNA-binding domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATTCAACTATTGGGGATATGGTGCATAAAGGCCCGAACCAGGCAAGT |
| Internal bar code: | GGGGAATAGTTCTGTAGCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2739 |
| LEAP-Seq percent confirming: | 38.4615 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCACGCTCTTGACTGTCA |
| Suggested primer 2: | TACACAGGCAGCTACCCTCT |