| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.055935 |
| Chromosome: | chromosome 6 |
| Location: | 3057969 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274950 | (1 of 1) K18953 - factor associated with neutral sphingomyelinase activation (NSMAF, FAN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGGCCTCGCTGGACCTGCACGACCCAGCCAACTTCAGGTGCGGGCCG |
| Internal bar code: | TCAGCCATTGGCAGGAGCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 248 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTGTAGGCGTGAGTGTG |
| Suggested primer 2: | CTCCCCTTGATCTGCTGCAA |