Insertion junction: CLIP2.055939_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.055939
Chromosome:chromosome 7
Location:986965
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g319350 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCTTTGGTCCTGCTTTGTCCGGGTGTACTTCGCCCCGCTGGGTTTGGG
Internal bar code:AACCCAGGTCGCTTACATACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3145
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCGCCATCGGATCACTC
Suggested primer 2:CCGCAATTAGGAGCACATGC