| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055943 |
| Chromosome: | chromosome 9 |
| Location: | 1173279 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401100 | (1 of 1) PTHR12560//PTHR12560:SF0 - LONGEVITY ASSURANCE FACTOR 1 LAG1 // LD18904P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCAGGAGCATCGGCTGGCGGTAGGCTAGAGAGGCCCAGCGTGGCGC |
| Internal bar code: | CGGACTTTGTCATATTTATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2327 |
| LEAP-Seq percent confirming: | 97.8495 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATTTACAGGTACGCAG |
| Suggested primer 2: | CAGGTGGTGCTTGATGAGGT |