Insertion junction: CLIP2.055947_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055947
Chromosome:chromosome 1
Location:194974
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g001300 ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAGATACGCACACACACACAAACACATGCACGCACACACACACGCACG
Internal bar code:CTGCTAGCTGTAGTTGCCTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:646
LEAP-Seq percent confirming:10.8108
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ACATTTGACACGCACACACG
Suggested primer 2:CAGAGGGATGGGGAGAAGGA