| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055948 |
| Chromosome: | chromosome 2 |
| Location: | 6349664 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387800 | FER1 | Pre-apoferritin; (1 of 2) K00522 - ferritin heavy chain (FTH1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGATGTTCTGGTTGGTCTTATCCACGGTCGCCAGCTCGCTCTGCACCT |
| Internal bar code: | GGAAATGCCGGTCGTCCTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2023 |
| LEAP-Seq percent confirming: | 75.4098 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTCGCGATTGAACTGAA |
| Suggested primer 2: | ACAGTTGAAGGCGATGGAGG |