| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055950 |
| Chromosome: | chromosome 7 |
| Location: | 3599781 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGTCGTTTATGCAAACGTAAGCGTTGGCTTGGCCATGTAGGGCTTTC |
| Internal bar code: | GTTCGGGGCGTGAAGTCGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4308 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTTTCAGTCCGCCTTGG |
| Suggested primer 2: | AACCCCACGTATTGCAGAGG |