Insertion junction: CLIP2.055963_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055963
Chromosome:chromosome 16
Location:1697520
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g654700 PHC61 Pherophorin-chlamydomonas homolog 61; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACACGGTAATGCTCACGTTGACCAGAGAGCCGCTGCTGCTGTTGGGA
Internal bar code:GATCCAGCAAGAGTAAGAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1486
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGGTAAGCGCAGGTAA
Suggested primer 2:CTAGCACCACCACTCGAAGG