Insertion junction: CLIP2.055972_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055972
Chromosome:chromosome 5
Location:160333
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g242550 RSP6,PF26 Radial spoke protein 6; (1 of 2) PF04712 - Radial spokehead-like protein (Radial_spoke) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGATCGGGGGAAGGGACAGAGGGCGAGGTCACCCTCAAACCAATGCCC
Internal bar code:ACATACTGTAACGACCGTGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2762
LEAP-Seq percent confirming:10.9756
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:73
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGTCCTCTGAGGAGTAGC
Suggested primer 2:GCATCCTTCAGTCCCGTTGA