| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055974 |
| Chromosome: | chromosome 3 |
| Location: | 6806918 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGCGATGGGACAAGAAAAGGGTGGCGAAGTCCCACGATCAGTGCTGC |
| Internal bar code: | CTGCTGGAGGGTATTATGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 45.9459 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCGCCGCTTCTATTTC |
| Suggested primer 2: | CCTTTGCTCCGACAGGGATT |