Insertion junction: CLIP2.055987_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055987
Chromosome:chromosome 5
Location:1333073
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g241655 (1 of 24) IPR001478 - PDZ domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGATCGTGGGAGCTATTGCACACAGCCTGCCTTCATCACCAGGACCC
Internal bar code:GTCGACCACACGGAGATGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5226
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:96
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:96

Suggested primers for confirmation by PCR

Suggested primer 1:CTCATCACCACCCACCTTCC
Suggested primer 2:CGACCCAACACAAACACGTC