Insertion junction: CLIP2.055991_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.055991
Chromosome:chromosome 1
Location:5571908
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039702 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGTCATGGAGCTGCTGCGCCCGGCGGACGGCTGGATGCCGCTGCAG
Internal bar code:TGGTGCGTGGTGGGGTGCTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2077
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGCGCACCATTATGTTG
Suggested primer 2:TGGACACTGAACACCATCCG