| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055998 |
| Chromosome: | chromosome 10 |
| Location: | 1588642 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429050 | XPG2 | DNA repair 5'-3' exonuclease; (1 of 1) K10846 - DNA excision repair protein ERCC-5 (ERCC5, XPG, RAD2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAAGCCGCAAATTGCTGCTTGTCAGCGCCGCTGTTGGTCTGTGCCAG |
| Internal bar code: | GATCGCGCGCTCCGAGTTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACAGCCAGAGTTGGTG |
| Suggested primer 2: | GCGGCGATGTGAAGAAGAAC |