Insertion junction: CLIP2.056001_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.056001
Chromosome:chromosome 7
Location:1959449
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325754 (1 of 3) 2.7.11.25//2.7.12.1 - Mitogen-activated protein kinase kinase kinase / MLTK // Dual-specificity kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATCCACCGCCACTCCCAACACCTACCGCCCCTCCCATCACCCACTCC
Internal bar code:ACCGTAAATTCTAGAACTTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3789
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACTCCATCGATGCCCAA
Suggested primer 2:CCAAACAGCGTCCACAAAGG