Insertion junction: CLIP2.056005_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.056005
Chromosome:chromosome 1
Location:490267
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800005 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGCCGCATTTGGGTTCGCCACGTTCCAATGGCCGGCTGTGAGTTGGG
Internal bar code:TAGGAATTCATATCGTAACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3371
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGTCATGTCGTAGGGCG
Suggested primer 2:AACTACGCCACCCAGAAGTG