Insertion junction: CLIP2.056015_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.056015
Chromosome:chromosome 10
Location:957191
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre10.g424600 (1 of 1) PF14771 - Domain of unknown function (DUF4476) (DUF4476) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGCGGTCGGTGATGAGGCAGAAGCACACCACCAGCACCTCCACGCGG
Internal bar code:GGGGACGAAGACGGAAAAGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:250
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGACACACAATCGCGTCTGA
Suggested primer 2:GCTTTTGTTTCGACGCCAGT