| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056026 |
| Chromosome: | chromosome 14 |
| Location: | 2178765 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622075 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAGCGACCTGGCTGCTCTTGGTGGGGCAGGGAGTGGGGGCGGGGCAG |
| Internal bar code: | CTTTACGCAGGTAGAATGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2715 |
| LEAP-Seq percent confirming: | 73.913 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTGCAGGAAGGTAGGT |
| Suggested primer 2: | TGAGGAGTTGGAGGAGGAGG |