| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056028 |
| Chromosome: | chromosome 7 |
| Location: | 6157976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356101 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTCGGAGCTGCAGTCCGCCTTCCTCACCTCCTGCCTCACCTACGCC |
| Internal bar code: | TAAGTGTTTACGAGGATTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1868 |
| LEAP-Seq percent confirming: | 76.6667 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTAGCTGTAGCCGTCAG |
| Suggested primer 2: | GGAGGAGGACCAGGAGGAAT |