Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | + |
Strain: | CLIP2.056034 |
Chromosome: | chromosome 11 |
Location: | 663351 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467616 | Dyf-13,IFT56,Ttc26 | (1 of 3) PF12895 - Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3); Intraflagellar Transport Protein 56 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAAAACATCGGGTCCGGGTCCTCGTGCTGCAACAGCTCCTTGTAGAT |
Internal bar code: | CGTCCCTGGTGGTGATATCAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 5810 |
LEAP-Seq percent confirming: | 91.6667 |
LEAP-Seq n confirming: | 11 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCATCTCTAGCCGTTACGCT |
Suggested primer 2: | GGGGAGGTAGGTGGAGATGT |