Insertion junction: CLIP2.056034_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.056034
Chromosome:chromosome 11
Location:663351
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467616 Dyf-13,IFT56,Ttc26 (1 of 3) PF12895 - Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3); Intraflagellar Transport Protein 56 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAAAAACATCGGGTCCGGGTCCTCGTGCTGCAACAGCTCCTTGTAGAT
Internal bar code:CGTCCCTGGTGGTGATATCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5810
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCTCTAGCCGTTACGCT
Suggested primer 2:GGGGAGGTAGGTGGAGATGT