| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056052 |
| Chromosome: | chromosome 9 |
| Location: | 5516093 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409100 | HEMD,UROS1,UPS1,UROS | Uroporphyrinogen-III synthase; (1 of 1) 4.2.1.75 - Uroporphyrinogen-III synthase / Uroporphyrinogen-III cosynthetase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCGCTGAATTTGTATAGAGCAAGACCTTGATACAAACCGAGAGGCCT |
| Internal bar code: | TCAAATGTAGATGGGTGTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1033 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCACCGTGTTACAGGTCG |
| Suggested primer 2: | GAGATCATGGGACGGAGCTG |