| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056062 |
| Chromosome: | chromosome 8 |
| Location: | 1598176 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365632 | PPP32 | Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGCGGCGGAGTCCTAGGCGGCGGAGTCTAGGTAAAAGGTTTGTTGCA |
| Internal bar code: | TCGGACTAGTATAAGAGTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1961 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTCTGAACCGGGGACTC |
| Suggested primer 2: | ACTTCTACACTGGCAACCCG |