| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.056064 |
| Chromosome: | chromosome 5 |
| Location: | 866310 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246200 | (1 of 2) PTHR24347//PTHR24347:SF159 - SERINE/THREONINE-PROTEIN KINASE // SERINE/THREONINE-PROTEIN KINASE STK11 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCCAGCGTGGCGAGACATTTAGGATGCGCCCTGTGCCAATCACCTTG |
| Internal bar code: | GCTCACCCCGGGCACTGTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCGCATACACAGTGCCG |
| Suggested primer 2: | CGTCTCATGCGCTATTTGCC |