| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056070 |
| Chromosome: | chromosome 12 |
| Location: | 150967 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484350 | CKIN beta-gamma,SNRKBG1 | regulatory subunit beta-gamma of snRK1 complex in Chlamydomonas; (1 of 1) K07200 - 5'-AMP-activated protein kinase, regulatory gamma subunit (PRKAG) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACATACGAAGAACGCAACTCATTGTTAGCAGTCGCCATTTTGTTACAG |
| Internal bar code: | AGACCGCAACGCTTTGATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2036 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACTTCCTTCCGGACCTG |
| Suggested primer 2: | TCAATGGATGCAAGGCTCGT |