Insertion junction: CLIP2.056078_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.056078
Chromosome:chromosome 9
Location:5764209
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g410900 NAR2,NIT8 nitrite transporter accessory protein; (1 of 1) PF16974 - High-affinity nitrate transporter accessory (NAR2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGCGCAACCAAGCAATGGATCGACAACCTTAATTGTTACATCGCGCT
Internal bar code:CTCGGATCTTTGTGAGTGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3789
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:153
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:153

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCTATTTCCGCAACACG
Suggested primer 2:CAAAACCGGACGTTCCACAC