Insertion junction: CLIP2.056078_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.056078
Chromosome:chromosome 9
Location:5764213
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g410900 NAR2,NIT8 nitrite transporter accessory protein; (1 of 1) PF16974 - High-affinity nitrate transporter accessory (NAR2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGTCAGTACGCAGCAGTCATGCACATGGGGGGGGCAATGCACCAAGC
Internal bar code:CTCGGATCTTTGTGAGTGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1304
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAACCGGACGTTCCACAC
Suggested primer 2:CGCTCTATTTCCGCAACACG