Insertion junction: CLIP2.056080_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.056080
Chromosome:chromosome 3
Location:637511
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145607 BET3 (1 of 1) IPR016721 - Bet3-like protein; Component of TRAPP complex outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGGGTGAGGGCTCCAGACGAGAACCCGGCCACACGCTGACCCACTGC
Internal bar code:CCCGGCTCATATGAGGGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1837
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TGAACATGGAGGTGGAGTGC
Suggested primer 2:TACGACGTGTGGGCATCAAA