Insertion junction: CLIP2.056081_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.056081
Chromosome:chromosome 4
Location:1124563
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g215950 CYN57 Cyclophilin 57; (1 of 1) K12737 - peptidyl-prolyl cis-trans isomerase SDCCAG10 [EC:5.2.1.8] (SDCCAG10) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACTCTTGCAGGCTTGGCACAAGAGTTTAGAAAGACGGTGAACGGAGA
Internal bar code:GACAAGATACCCACGCCTTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4141
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:122

Suggested primers for confirmation by PCR

Suggested primer 1:TCACACGGACTTTGACCTCG
Suggested primer 2:ACACGATTCAACTGGACGCT