| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056093 |
| Chromosome: | chromosome 14 |
| Location: | 557692 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611517 | (1 of 3) K07393 - putative glutathione S-transferase (ECM4) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCAGGGATCATGCCCAAGTGCCAAAACACAATCGTTGCCATCGTTGC |
| Internal bar code: | TTGACTTTTCATGTACTTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAGAACCTCGCTTAGCA |
| Suggested primer 2: | TTGCGTTGTGGCGTAAGTTG |