| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056104 |
| Chromosome: | chromosome 4 |
| Location: | 2679371 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223250 | LCIE,LCIE1 | Low-CO2 inducible protein E | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGATGATGTCCATACATCCGGTCCGCTCTTCTCTTTCGCTCAATCCTG |
| Internal bar code: | GTTCAAGGGTCTTTGCTAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1975 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTGACTTGCGAGCTTG |
| Suggested primer 2: | GGCTGCTCAGATCACCAAGT |