| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.056111 |
| Chromosome: | chromosome 3 |
| Location: | 7491442 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205550 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTTAAGGGTGCCTGGGGCCAGGGGCCAGAGCGAGGCGCCCGCGCCAC |
| Internal bar code: | ATCGGAATTTCAGTTAAACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1293 |
| LEAP-Seq percent confirming: | 51.6667 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGCCTCGTATACGGTAA |
| Suggested primer 2: | CTTGCAAATGCCACCGAACA |