| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056115 |
| Chromosome: | chromosome 9 |
| Location: | 4415947 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400478 | DIV31 | phosphatidylinositol 4-phosphate 5-kinase; (1 of 1) PTHR23086:SF8 - PROTEIN PPK-2 | 3'UTR |
| Cre09.g400516 | (1 of 6) K04710 - ceramide synthetase (CERS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTCGGCCTGTCCCTTCACGGTCCCTTCTTCACACAAGCACACGGATA |
| Internal bar code: | GGAGGATGTTACCCAATCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1949 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGCTTGGTGGAGAAGG |
| Suggested primer 2: | TGATGGAGGGAGGAGGACTG |