| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056118 |
| Chromosome: | chromosome 14 |
| Location: | 1175763 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615450 | mL117,OPR132,MRPL117 | mitochondrial ribosome protein mL117, Chlamydomonas-specific | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTAGTACATAGATTTTGTTACAGACACAGGACAACGGGTGCCCTGCA |
| Internal bar code: | TTCGAACGATTGCGTTCAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2527 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTAGCTGTCACTGGCA |
| Suggested primer 2: | CGCGTGCGTTGAACAACTTA |