| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056128 |
| Chromosome: | chromosome 17 |
| Location: | 1003661 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703176 | MSD5 | (1 of 5) K04564 - superoxide dismutase, Fe-Mn family (SOD2); Mn superoxide dismutase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATTTATGGGGGTCCTGGTTATCAGGTGCTTTAAGCTGCAATGGAGGA |
| Internal bar code: | GTAGCAAGGGGCTGAGAGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 978 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTTGTGCGTGAGTAGGG |
| Suggested primer 2: | AAAAACTTTGCGAGCGGTCC |