| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056151 |
| Chromosome: | chromosome 16 |
| Location: | 1974513 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656400 | SQD1 | (1 of 1) 3.13.1.1 - UDP-sulfoquinovose synthase / Sulfite:UDP-glucose sulfotransferase; UDP-sulfoquinovose synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGCTGGATGGCGGAATAAACACACCCGGGAAAGGCATATGCGAAGCG |
| Internal bar code: | TTACTCCTCTGTGGACGTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTATGTACCGGATTCGC |
| Suggested primer 2: | CGTAAGTCCGCTAGCGCTTA |