Insertion junction: CLIP2.056156_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.056156
Chromosome:chromosome 7
Location:5545343
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre07.g351750 (1 of 1) K14823 - rRNA-processing protein EBP2 (EBP2, EBNA1BP2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCACACCCACCCATCCCACACGCATACACACGCGCTCCGCGCACACA
Internal bar code:AGCGTCTCTTGTTAGAAGTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:224
LEAP-Seq percent confirming:35.7143
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGACAGACTGTGTGGAG
Suggested primer 2:CCTGTTCTCCCACACATCCC