| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056160 |
| Chromosome: | chromosome 10 |
| Location: | 4339966 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449800 | (1 of 1) PF04657 - Putative inner membrane exporter, YdcZ (DMT_YdcZ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCCATCACCACACAAACTACAACAGCCATGCACAAACGATTGCTTT |
| Internal bar code: | AGTGACTAGAAAATTGTGAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1948 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAATCGCTAGTGGGTGCA |
| Suggested primer 2: | ACAGCGATCATCCCCGATTC |