| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056168 |
| Chromosome: | chromosome 2 |
| Location: | 1592603 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085000 | (1 of 1) PTHR22847//PTHR22847:SF423 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGTCAAGCAGAGCGCATGCCCCGGCTTGCTCATTCTTTGCCGCTCC |
| Internal bar code: | ACAGCAGAACCAACCATGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 63.1579 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGATTTAGGGGAGCAAC |
| Suggested primer 2: | CTGTTGTCGTACACTGGGCT |