| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056198 |
| Chromosome: | chromosome 15 |
| Location: | 1267387 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g801726 | NCL65 | (1 of 3) 6.1.1.9 - Valine--tRNA ligase / Valyl-tRNA synthetase; Nuclear Control of chloroplast-Like 65 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATATGGTCAGGGCGGAATGAAGTAAGGCTAGAGCTGCCACAGTCGTCAT |
| Internal bar code: | TGCAGCGGCTGCGATTAATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3203 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGCAAGTCCTCCAGAG |
| Suggested primer 2: | ACAGGAGCGGTCGTTAGTTG |