| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056235 |
| Chromosome: | chromosome 7 |
| Location: | 2986136 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333350 | 5'UTR | ||
| lncRNA_TCONS_00245377 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCACAATGTGCAATAGAGGCAGATGGCAAGGACGGTGAGATGAGTGC |
| Internal bar code: | TGTAGTATCTATAAGGGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2253 |
| LEAP-Seq percent confirming: | 97.2603 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTCTCCGTCCGGCATAG |
| Suggested primer 2: | GTCAGCGTCTGAGCCAGAAT |