| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056241 |
| Chromosome: | chromosome 6 |
| Location: | 8269732 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306850 | HEL31 | (1 of 1) K18655 - ATP-dependent RNA helicase DDX19/DBP5 [EC:3.6.4.13] (DDX19, DBP5); DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGGCGCAAGGGCCACGGGTGCACAGCCGCGGGGGTGGCCACTGGCC |
| Internal bar code: | TATTCATTGTGACGCTCTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2962 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCTACTCGCATTCCCCT |
| Suggested primer 2: | GGGATGACGAGGACGAGTTC |