Insertion junction: CLIP2.056258_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.056258
Chromosome:chromosome 4
Location:3683302
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g228950 3'UTR
Cre04.g229000 IP5-2K,IPK1 (1 of 2) 2.7.1.158 - Inositol-pentakisphosphate 2-kinase / IP5 2-kinase; Inositol polyphosphate kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGTGACACCGCGCGCGCATCCTGCTGCTCATCCTGGCCGTTCTTCATC
Internal bar code:CTTTCGATTTCGTGTTGGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3768
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:94

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCGTCAGCTCTGTATCCG
Suggested primer 2:AACTCCTCCCCCTCCTCATC