| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056259 |
| Chromosome: | chromosome 7 |
| Location: | 6100826 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355500 | (1 of 2) PF03226 - Yippee zinc-binding/DNA-binding /Mis18, centromere assembly (Yippee-Mis18) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTCACCGCCACCTCCGCACTCACCTGTCCGGTCCCCCACCTCGCCT |
| Internal bar code: | TTAAACGGCTACGTAGATTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATACGCCAGCTCCTGAG |
| Suggested primer 2: | TATTTCAGAGCTGGCCGCAA |