| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056261 |
| Chromosome: | chromosome 12 |
| Location: | 2685357 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505150 | OPR52 | (1 of 781) IPR000104 - Antifreeze protein, type I; OctotricoPeptide Repeat protein 52 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGCGGTGCGCCGCTGCCGACATAGTGCTGAATTGTTTCAAGCTTCG |
| Internal bar code: | CGGTCAGTATACCCATAGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1591 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATGAGCTGGCTCGGTAG |
| Suggested primer 2: | TGGTGCTTACGGAAGGTGTC |