| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056276 |
| Chromosome: | chromosome 12 |
| Location: | 6989485 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560800 | (1 of 2) K14664 - IAA-amino acid hydrolase [EC:3.5.1.-] (ILR1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAGAGCGGCAGACCATATATAACATTACACTGTCACCATTCACGCTGA |
| Internal bar code: | GGTGGGAGCTCGTCATATGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5195 |
| LEAP-Seq percent confirming: | 98.913 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTAGGTGTACGGTATGC |
| Suggested primer 2: | CTTAACCACCACACCGGACA |