| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056277 |
| Chromosome: | chromosome 10 |
| Location: | 2724214 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437900 | SPL31 | (1 of 1) PTHR17204//PTHR17204:SF29 - PRE-MRNA PROCESSING PROTEIN PRP39-RELATED // SUBFAMILY NOT NAMED; Pre-mRNA splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCCAGCTCTCAAGTCTACGTCAGCATTCTGCTAAGATCGCTTGATTG |
| Internal bar code: | ACAACGTTAACCCCTGGCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1797 |
| LEAP-Seq percent confirming: | 80.6452 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGACCTTCATGCCTTCAT |
| Suggested primer 2: | CACAGCAAGGAGGAGGTGAG |