| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.056282 |
| Chromosome: | chromosome 2 |
| Location: | 1290200 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082350 | CUTA1,CUT1 | (1 of 1) K03926 - periplasmic divalent cation tolerance protein (cutA); Copper-binding protein CutA | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGACATTGACGCACCAGTGTTAGGCACGGACAGGGAAAAGAACCAGGC |
| Internal bar code: | ACGGTGGTCTAGGTAGCTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1507 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCAGTGGAGAGGCAGCTG |
| Suggested primer 2: | ACCCCACATAGTTCTGCTGC |