Insertion junction: CLIP2.056296_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.056296
Chromosome:chromosome 1
Location:277850
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAACCCTATTTTTCTACTATAGGGAAAGAAAAATAGAAAGTAATAATA
Internal bar code:ACTGTCCACCGTCACGCTTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:393
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAACTTACTGCTACACGCGC
Suggested primer 2:CAGCACAGTTGTCAACAGCC